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Posts Tagged ‘B08 IQP’

This is a general outline of the methodology used by the B08 Origins Team, when performing their DNA testing.

Before testing can begin, permission to use humans as subjects must be obtained. The B08 group received this clearance from the WPI Institutional Review Board, by filling out the appropriate paperwork and submitting it for review.

Dr. David Comas outlined a general protocol for the collection of DNA samples in and around Venice. He stipulated that all of the participants should be male, and above the age of 18. They should not be related, directly (no siblings, fathers/sons, uncles/nephews, etc), and the grandparents of the participants should be from the same geographical area. Once these stipulations were met, each individual was to read and sign the informed consent paperwork. He also suggested that neighboring regions, such as Friuli Venezia Giulia, Trentino Alto, Adige, Lombarida and Emilia Romagna (etc.) be tested as well.  

The informed consent paperwork outlines the purpose of the project, collection procedure, storage of the sample, potential risks and benefits for the individual, and declaration of confidentiality.

Once the paperwork is signed, the individual is then subject to the cheek swab, administered by one of the IQP group members. The swab is opened, and removed, carefully, so as not to touch the collection tip. The collection tip is then scraped firmly against the inside of the participant’s cheek (roughly about 5-6 times, or 10 seconds). The tip is then ejected, using the plunger at the end of the handle, into the appropriately labeled 2mL microcentrifuge tube. Each participant then receives an information card that has their sample number and directions to obtain information on their sample via the Genographic website.

There is not much mention of how they targeted their subjects. Further research must be done to determine the best way to do so.

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